Salinity Tolerance, Plant Growth, and Nutrient Partitioning in Three Mexican Huauzontle (Chenopodium berlandieri subsp. nuttalliae) Genotypes Under NaCl Stress

Huauzontle (Chenopodium berlandieri subsp. nuttalliae) is a pseudocereal native to Mesoamerica, traditionally consumed as a nutrient-rich food and characterized by its adaptability to adverse environmental conditions, including salt stress. This study evaluated the effects of four NaCl concentrations (0, 100, 200, and 300 mM) on plant morphology and nutrient concentrations (N, P, K, Ca, Mg, Fe, Cu, Zn, Mn, and B) and Na in leaves, stems, inflorescences, and seeds of three native huauzontle genotypes. The experiment was conducted using a completely randomized design with a split-plot arrangement and 12 replications. Applications of 200 and 300 mM NaCl delayed harvest and reduced seed weight, while plant height, fresh and dry biomass of stems, leaves, and inflorescences were progressively decreased as NaCl concentrations increased. Orthogonal partial least squares discriminant analysis (OPLS-DA) clearly differentiated genotypes and grouped NaCl treatments into distinct clusters, revealing different nutrient partitioning patterns among plant organs. Nutrient accumulation varied according to organ and salinity level; leaves showed reduced N, K, Ca, Mg, and Fe concentrations, whereas Cu and Mn concentrations increased. Huauzontle exhibited high salinity tolerance, maintaining growth and development at NaCl concentrations up to 300 mM. These findings highlight the potential of huauzontle as a resilient and nutritionally valuable crop for cultivation under saline conditions.