Untargeted Serum Proteomics in the Fontan Circulation Reveals Three Distinct Molecular Signatures of Fontan Physiology with CYB5R3 Among Key Proteins
Abstract
The total cavopulmonary anastomosis (Fontan procedure), a palliative procedure for single-ventricle congenital heart disease, improves survival but is associated with progressive multiorgan complications and high long-term morbidity. Prior blood-based proteomic studies in adults have been limited to targeted antibody-based panels or focused on methodological comparisons. Systemic molecular alterations in younger, clinically heterogeneous patients, particularly in untargeted pathways, remain incompletely characterized. Serum samples from 48 Fontan patients and 48 age- and sex-matched healthy controls were analyzed using mass spectrometry with TMT labeling. 2228 proteins were quantified, of which 124 were significantly differentially abundant (fold change > 1.5 or
Fields of Study & Subjects
Congenital Heart Disease Studies
Congenital heart defects research
Cardiac Fibrosis and Remodeling
Publication details
- Journal / source
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Volume
- 27
- Issue
- 3
- Pages
- 1220–1220
- Publication date
- 26 January 2026
- Language
- EN