Ultrasonic Treatment Suppresses Biofilm-Mediated Larval Settlement of Mussels: A Pilot Study

Marine biofouling significantly impacts vessel operational efficiency, with mussel species being particularly problematic due to their rapid settlement on biofilm-covered surfaces. This pilot study presents the first explicit test of whether ultrasonic treatment can disrupt the biofilm–larva interaction pathway that facilitates mussel settlement. The study evaluated ultrasonic treatment (28 kHz) as a preventive antifouling strategy targeting the mixed microbial biofilm-mediated settlement pathway of Mytilus edulis. A controlled laboratory experiment compared settlement rates on biofilm-conditioned (2.5-week mixed microbial biofilm development) and unconditioned steel plates with and without ultrasonic treatment. Under control conditions, biofilm presence increased mussel settlement odds by 49-fold (p < 0.001). Ultrasonic treatment eliminated this biofilm enhancement, maintaining settlement at baseline levels (odds ratio: 1.3, p = 0.84). The mechanism remains unclear but may involve biofilm disruption, larval behavioral avoidance, or interference with chemical cues. While limited replication (n = 2, temporal replicates, one tank per treatment per replicate) constrains statistical power and inference, the large effect size and consistency across replicates warrant additional investigation. If confirmed by increased replication and mechanistic studies, ultrasonic treatment could provide sustainable antifouling protection without chemical discharge.