Enzymatic Degumming of Arachidonic Acid Oil Using Immobilized Phospholipase A1 on Hollow Double-Layer Mesoporous Silica Nanoparticles

Molecules 2026 Open access

Read full text
Authors: Xuefeng Li, Wenlong Yuan, Lin Mei, Yufei Zhang, Lei Ping, Mingming Zheng, Shengjiao Yu, Xi Ping Du, Jun Wei
DOI: 10.3390/molecules31050816
Cited by: 1
References: 42

Abstract

This study explores the application of immobilized phospholipase A1 (PLA1) on hollow double-layer mesoporous silica nanoparticles (PLA1@NH2/C8-HdlMS) for the degumming of crude arachidonic acid (ARA) oil for the first time. The immobilized enzyme was comprehensively characterized, and the reaction conditions were optimized via single-factor experiments. Under the optimized conditions (enzyme dosage 0.3% w/w, 35 °C, water addition 3%, and reaction time 90 min), PLA1@NH2/C8-HdlMS achieved a remarkable phosphorus removal rate of 97.9%, reducing the phosphorus content from 441.21 mg/kg to 9.29 mg/kg in 90 min (well below the food-grade standard of

Fields of Study & Subjects

Edible Oils Quality and Analysis Enzyme Catalysis and Immobilization Biodiesel Production and Applications

Publication details

Journal / source
Molecules
Publisher
MDPI AG
Volume
31
Issue
5
Pages
816–816
Publication date
28 February 2026
Language
EN