Evaluation of Antibacterial Substances Through a 32 Factorial Design for the Treatment of Poultry Slaughterhouse Wastewater

Poultry farming is important from a food point of view, as it provides products for the population. Waste resulting from poultry farming and processing is environmentally sensitive, which is why treatment technologies are crucial for sustainability. Conventional pre-treated wastewater originating from the poultry sector, combined with innovative methods of using substances with antimicrobial effects constitute another approach to eliminating health risks. This research aimed to evaluate the behavior of bacterial strains isolated from water samples treated by reverse osmosis (RO), ultrafiltration (UF), and ultraviolet disinfection (UV). To choose our antimicrobial substances, we surveyed the population using the Google Forms platform and the official questionnaire. Using a factorial design, the concentration of antibiotic or antiseptic, as well as the duration of their action on bacterial strains isolated in pure culture, was varied. The optical densities of bacterial cultures reflect the efficiency of treatments with antibacterial or antiseptic substances. Factorial design, corroborating the experimental results, led to the following: 4.84 mg·L−1 antibiotic for 7.6 h and 4.23 mg·L−1 chloramine for 16.23 h. The results obtained from mathematical modeling correspond to a decrease in the bacterial population. In perspective, combining purification technologies with antimicrobial substances, adapted to the volume of water through mathematical modeling, will reduce the risk of microbiological contamination, with bacterial recovery rate values ranging between 0.58 and 1.21 in the case of antiseptic using, and between 0.60 and 0.92 in the case of antibiotic using.