Multi-Feature Fusion and Optimization for Micropterus salmoides Tracking and Body Length Monitoring in Complex Aquaculture Environments

To achieve non-contact and continuous monitoring of body length in Micropterus salmoides and overcome the stress damage and subjective error associated with traditional manual measurement, this paper proposes an improved YOLOv8-based multi-target tracking framework for intensive recirculating aquaculture systems. The system employs a geometric measurement framework based on monocular vision that achieves conversion from pixel coordinates to actual body length through camera calibration, water-surface refraction correction, and pose projection correction. Under a collaborative optimization framework integrating detection and tracking, the model incorporates multi-scale feature enhancement, lightweight re-identification (ReID), and a robust data association mechanism, which improves system stability under conditions of high fish density, variable illumination, and turbid water. A shallow feature fusion path is introduced to enhance small-target perception, and a MobileNetV3_ReID model is adopted to extract highly discriminative appearance features, which improves identity consistency while maintaining model compactness. In the data association stage, a hybrid cost matrix integrating IoU, cosine similarity, and motion consistency is constructed, and optimal matching is realized through the Hungarian algorithm. Dynamic threshold adjustment and an exponential moving-average feature-update strategy are introduced to effectively suppress identity switching. Experiments were conducted on an overhead video dataset of Micropterus salmoides collected at a recirculating aquaculture system facility. The results show that the proposed method achieves 82.7% mAP50 while maintaining a real-time throughput of 88 FPS, with MOTA reaching 76.9% and IDF1 reaching 81.5%—the latter representing an improvement of 3.2 percentage points over BoT-SORT and 5.3 percentage points over the YOLOv8 baseline tracker. The number of identity switches (IDSW) decreased from 89 in the baseline configuration to 39, a reduction of 56.2%. Crucially, these component-level improvements translate into a body length error (BLE) of 5.2 ± 1.8% (MAE = 1.35 cm, Pearson r = 0.972), representing a 38.8% improvement over the baseline BLE of 8.5% and satisfying the 5–10% tolerance required for aquaculture growth monitoring. Ablation analysis confirms that both detection enhancements (contributing −1.3% BLE) and tracking optimizations (contributing −2.0% BLE) are necessary to achieve this application-level accuracy.