Seminal cryopreservation with nano supplementation as an antioxidant agent: a literature review

Seminal cryopreservation is a widely used technique in assisted reproduction. The technique is used for humans and animal samples to preserve cellular genetic material for long periods of time in low temperatures. However, this procedure can cause cellular damage by increasing the reactive oxygen species (ROS), which may negatively affect DNA and acrosome integrity, sperm motility and mitochondria function. Therefore, research are necessary to prevent such damage. Recent studies have investigated supplementation of cryopreservation media with nanoproducts, mainly with antioxidant potential, aiming to reduce ROS and improve sample quality after thawing. This literature review analyzed 13 studies that used nanotechnology for this purpose. The articles were arranged on a table with their information and then some frequent topics were discussed.