Replication data for "Carbonic anhydrase boosts multi-step CO2 conversion: A synergistic approach of co-immobilized enzymes for CO2 valorization"

Dataset related to the immobilization of three microbial variants of carbonic anhydrase (CA) on the Ni2+-ReliZyme carrier, their contribution to CO2 reduction to formate by FDH, rational design and optimization of the trifunctional biocatalyst from cell lysates by evaluating different BhCA loadings, different BhCA concentrations in CO2 reduction, different GC-GlyDH loadings in the final biocatalyst, evaluation of the effect of BhCA in the multi-enzymatic system, operational stability, reusability of the biocatalyst over five reaction cycles, and performance metrics.