Time-dependent metabolomic profile following repeated non-penetrating mild blast traumatic brain injury on male C57BL/6J-Tyr mice

STUDY PURPOSE: Blast-induced traumatic brain injury causes on-going deficits in brain metabolism. The dataset is genereated from a study designed to investigate how repeated mild blast traumatic brain injury (rmbTBI) alters cortical metabolites over time, focusing on acute (1 day) and chronic (30 days) phase post-injury. DATA COLLECTED: A total of 24 male C57BL/6J-Tyr mice (9 weeks old) were randomly assigned to sham or rmbTBI groups, with n = 6 animals per group at each time point. rmbTBI was produced using the open-ended McMillan Blast Device and animals underwent two separate blast exposures separated by 24 hours. Each exposure was generated using compressed helium to yield a target peak static overpressure of ~11 psi with pressure measured using a sensor adjacent to the animal’s body (see metadata for exact recorded pressures). During exposure, mice were positioned laterally and secured in a mesh sling to limit movement and were anesthetized with isoflurane for both blast procedure and euthanasia. At designated time points, cortical tissue from the right hemisphere was rapidly extracted, flash frozen, and analyzed using untargeted liquid chromatography–mass spectrometry (LC-MS) metabolomics. Metabolites were extracted via methanol precipitation, separated using hydrophilic interaction liquid chromatography (HILIC), and analyzed in both positive and negative ion modes. Data were normalized to tissue mass and analyzed using MetaboAnalyst (p < 0.05). DATA USAGE NOTES: