Impact of Ruthan date extract in alleviation of cypermethrin induced pancreatic toxicity via SiRT-1 and Cyto-c in rats

Cypermethrin (CYPn) is a commonly used pesticide in agriculture and houses for insect's control.We evaluated the pancreatic toxicity in rats exposed to CYPn and the protective efficacy of Ruthana date extract (RDE) as against toxicity.In the current study, male Wister albino rats were allocated into five groups; (8 rats/group).Group I: Control, while rats in groups II-V were daily given 100 mg/ kg CYPn (1/10 LD50) orally for 4 weeks.Groups III, IV and V; rats were supplemented in the same time 100, 200 and 300 mg RDE/kg respectively.Data obtained showed that, phytochemical analysis of RDE showed its high content of catechin, naringenin and apigenin as major flavonoids content.Also, rats given CYPn showed a significant elevation in fasting blood glucose, glycated hemoglobin (HA1c), malondialdehyde (MDA) and interleukin-6 (IL-6) levels while the insulin and GSH levels were significantly reduced (p< 0.001) compared with control.On the other hand, the activities of antioxidant enzymes (GST, GSPx and SOD) were increased in rats injected with CYPn versus control.In addition, CYPn increased HOMA-IR, cytochrome c (Cyto-c), and reduced Sirtuin 1 level.Rats treated with RDE restored these abnormalities with dose-dependent.Flavonoids (catechin, naringenin and apigenin) from RDE showed a protective effect against toxicity induced by CYPn via reduction of antioxidants activities, sirtuin 1, insulin sensitivity, reduction Cyto-c release and inflammatory mediators.It was concluded that, the active ingredients of RDE is promising in developing a safe protective agent against pancreatic toxicity and incidence of diabetogenesis induced by CYPn.