Some indicators of glycome in various forms of multiple sclerosis

Aim: identification of protein glycosylation features in various clinical forms of multiple sclerosis. Material and methods. We studied the indicators of glycome, viz. interleukin-6 (IL-6) and P-selectin glycoprotein (SELP) in 135 patients with various forms of multiple sclerosis (MS): relapsing-remitting MS (RMS) - 71, secondary progressive MS (SPMS) - 49, primary progressive MS (PPMS) - 15 patients. An ELISA Multiscan FC analyzer was used at 450 nm using appropriate diagnostic kits. Statistical processing was performed using to the Mann - Whitney criterion. Multiple comparisons of groups of different MS course were performed using the Kruskal - Wallis test. Correlation analysis was performed based on Spearman’s rank correlation coefficient. Statistical reliability of conclusions was determined at the 5% level of significance. Results. This study attempted to identify the relationship between IL-6 and SELP levels and MS shape, duration, and severity, revealing only a significant association of SELP with disease duration for RMS. There were no data on the relationship of these indicators with the age and sex of patients. Conclusions. The studies carried out show a certain specificity of changes in glycosylation of proteins in multiple sclerosis, which makes it possible to use them as markers for diagnosing various forms of multiple sclerosis and similar diseases. Despite the fact that the study showed a significant association only in SELP and only with the duration of the disease in relapsing-remitting MS, it is possible to obtain additional results with an increase in the number of patients included in the study, as well as with the inclusion of other glycome parameters in the study.