Microscopic and Molecular Diagnosis of Ascaris lumbricoides in Human of Babylon Governorate

Human ascariasis is caused by the soil-transmitted helminth (STH) Ascaris lumbricoides. The disease found all across the world, with particularly high incidence in tropical and subtropical regions, as well as Iraq. In the current investigation, 200 stool samples were collected between January 2024 and January 2025 from four distinct in the province of Babylon in order to isolate and identify Ascaris lumbricoides. 200 stool samples for traditional diagnosis by (salt floatation, Kato-Katz technique) and 100 samples for Molecular Diagnosis. The prevalence rate was 13% (26/200), 21% (21/100) by traditional and Molecular Diagnosis respectively, according to sex was higher in males (28%) than females (14%) and the age groups 5-10 recorded the highly in male and female (50%), (42.8) , the area of study recorded heights in Al –Kifl was 28% ( 7/25), seasonal show Summer had the greatest infection rate (32%) and winter had the lowest (12%), Ten sequences analysis each belonging of Ascaris lumbricoides Phylogenetic tree (PX418260 ,PX418261, PX418262, PX418263, PX418264, PX418265, PX418266, PX418267, PX418268 and PX418269).