Silver Nanoparticles of Moringa oleifera - A Comprehensive Study on Antioxidant, Anti-Inflammatory and Cell Line Based Anticancer Properties.

Abstract Silver nanoparticles (AgNPs) have gained considerable attention as potential therapeutic agents owing to their notable antioxidant, anti-inflammatory, and antitumor activities. The present study focuses on the eco-friendly synthesis of AgNPs utilizing Moringa oleifera flower extract and explores their biological properties. The formation of AgNPs was confirmed by UV–visible spectroscopy, which exhibited a characteristic surface plasmon resonance peak at 430 nm. The antioxidant potential of the synthesized AgNPs was evaluated using standard in vitro assays, demonstrating effective free radical scavenging activity with a maximum inhibition of 54.63 % inhibition at a concentration of 1000μg/ml, indicating their ability to mitigate oxidative stress implicated in chronic disorders such as diabetes and cardiovascular diseases. Additionally, the AgNPs exhibited significant anti-inflammatory activity, achieving 64.70 % inhibition at 100μg/ml, suggesting their therapeutic relevance in inflammatory conditions. The anticancer activity of the synthesized AgNPs was evaluated using the MTT assay against the MCF-7 human breast cancer cell line. The results, based on absorbance measurements, revealed a concentration-dependent effect on cell viability. At the lowest concentration tested (7.8 µg/ml), MCF-7 cells exhibited higher cell viability (63.37%), indicating minimal cytotoxicity. However, with increasing concentrations of AgNPs (1000μg/ml), a gradual decrease in absorbance and cell viability (31.42%) was observed, reflecting reduced metabolic activity and enhanced cytotoxic effects. These findings provide a valuable foundation for future preclinical investigations and suggest that green-synthesized AgNPs could contribute to the development of novel therapeutic strategies for cancer and other chronic diseases.