Potential Activity of Fungal Endophyte Lasiodiplodia Irregularis Isolated from Guiladina Bonduc as a Source of Antimicrobial Compound

The endophytes, are the microorganism which inhabit in distinct parts of the plant without showing any symptom. Fungal endophytes are treasury of therapeutically significant bioactive compounds. Guilandina bonduc is a divine tree, and important indigenous medicinal plant. It has been utilized in medicinal systems of Ayurveda, Siddha and Unani for treating several diseases. The aim focuses on isolation of fungal endophyte Lasiodiplodia irregularis from the plant part of Guilandina bonduc. It is a store house of endophytes, that produces magnificent therapeutic compounds. Thin layer chromatography (TLC) and Gas Chromatography-Mass Spectrometry (GCMS) were used to determine the chemical composition of fungal extract of endophytes. Our analysis of fungal extract through TLC and GC-MS shows the presence of 25 different type of compounds. The qualitative survey of methanolic solution of endophytic fungus confirms for terpenoids, steroids, carbohydrates alkaloids, flavonoids, tannins, and amino acids. Antimicrobial activity performed against Gram-negative and Gram-positive bacteria by disc diffusion method inhibited the growth of bacteria.