Effects of Whole-Body Vibration Training on Neurological Function and Ferroptosis in A Rat Model with Cerebral Ischemia-Reperfusion Injury

ObjectiveTo observe the effects of whole-body vibration training on the neurological function and ferroptosis of rat model of cerebral ischemia-reperfusion injury (CIRI), and to explore the mechanism by which whole-body vibration training ameliorates CIRI.MethodsForty SPF-grade male Sprague-Dawley (SD) rats were randomly divided into the sham operation group (n=10) and the model establishment group (n=30) according to the random number table method. The model group rats were established using the modified Zea-Longa wire embolization method to create a CIRI model. The dynamic monitoring of brain blood flow changes before model preparation, during wire embolization insertion, and after wire embolization removal were conducted using the laser speckle blood flow imaging system to confirm the manifestations of cerebral ischemia-reperfusion. The Zea-Longa neurological deficit score was used to evaluate whether the model was successfully established. Twenty rats with successful modeling were randomly selected and randomly divided into the model group and the whole-body vibration training group according to the random number table method, with 10 rats in each group. The rats in the whole-body vibration training group received whole-body vibration training with a frequency of 20 Hz starting from the second day after the model establishment. The training lasted for 15 minutes each time, twice a day, and was conducted continuously for seven days. The rats in the sham operation group and the model group received no intervention. After the intervention, the Zea-Longa neurological deficit score was assessed. 2,3,5-triphenyltetrazolium chloride (TTC) staining was used to detect the cerebral infarction area. Hematoxylin-eosin (HE) staining and Nissl staining were employed to observe pathological changes in the cerebral infarction area. Enzyme-linked immunosorbent assay (ELISA) was utilized to measure the levels of malondialdehyde (MDA), glutathione (GSH), and ferrous ions (Fe2+) in the cerebral infarction area. Immunofluorescence staining was applied to detect the protein expression levels of glutathione peroxidase 4 (GPX4) and solute carrier family 7 member 11 (SLC7A11) in the infarction area.ResultsCompared with the sham operation group, the neurological function deficit score in the model group was higher (P