VALIDATION OF A COST-EFFECTIVE UV-VIS SPECTROPHOTOMETRIC METHOD FOR MALONDIALDEHYDE QUANTIFICATION IN RAT JEJUNUM: APPLICATION IN GASTROPROTECTIVE STUDIES

Rigorous analytical method validation is fundamental to ensuring data accuracy and reproducibility in oxidative stress research, particularly when quantifying biomarkers in complex biological matrices. To address this, we developed and validated a UV-Vis spectrophotometric method for quantifying malondialdehyde (MDA) in intestinal tissue. Using optimized wavelength selection (532 nm) and centrifugation (8,000 rpm), the method demonstrated excellent linearity (R² = 0.998), sensitivity (LOD = 0.15 ?g/mL; LOQ = 0.45 ?g/mL), and precision (%RSD < 5%), in compliance with the ICH Q2(R1) guidelines. When applied to the jejunum of ketorolac-treated rats, the method confirmed elevated MDA levels, validating its utility for the preclinical screening of gastroprotective agents. This protocol offers a cost-effective alternative to HPLC with 98% concordance, making it highly suitable for resource-limited settings. Keywords: UV-Vis spectrophotometry, malondialdehyde, oxidative stress, method validation, ketorolac