Ultracentrifugation-Based Isolation of Legionella pneumophila Outer Membrane Vesicles
Abstract
Source: Jung, A. L., et al. Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages. J. Vis. Exp. (2017)This video demonstrates the isolation of outer membrane vesicles (OMVs) from Legionella pneumophila using sequential centrifugation, sterile filtration, and ultracentrifugation. The purified OMVs, enriched in membrane proteins and lipopolysaccharides, are suitable for host-pathogen interaction studies.
Fields of Study & Subjects
Legionella and Acanthamoeba research
Bacterial Infections and Vaccines
Immune Response and Inflammation
Publication details
- Journal / source
- Journal of Visualized Experiments
- Publication date
- 3 February 2026
- Language
- EN