Live Imaging of Host-Pathogen Interactions in Zebrafish Embryos Infected with Mycobacterium abscessus

Source: Bernut, A., et al. Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos. J. Vis. Exp. (2015)This video demonstrates a protocol that uses transgenic zebrafish embryos expressing fluorescent macrophages and infected with fluorescent Mycobacterium abscessus rough strain to visualize host-pathogen interactions. It reveals how extracellular cording enables immune evasion, promoting bacterial survival and host cell damage.