JoVE Video Dataset

2026 Dataset

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DOI: 10.3791/24423-v
Cited by: 0

Abstract

10 Views. Source: In, J. G.,et al. Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium. J. Vis. Exp. (2019). This video demonstrates the procedure for culturing colon-derived epithelial cells on a collagen-coated Transwell system to generate a mucus-producing monolayer that models the intestinal barrier and enables host–pathogen interaction studies.

Fields of Study & Subjects

Gut microbiota and health Gastrointestinal motility and disorders Escherichia coli research studies

Publication details

Publication date
27 February 2026
Language
EN
Document type
Dataset