Detection of Saccharibacteria in Binary Culture Using Polymerase Chain Reaction
Abstract
Source: Collins, A. J., et al., Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity. J. Vis. Exp. (2021)This video demonstrates the use of Polymerase Chain Reaction (PCR) to detect Saccharibacteria in a binary culture with Actinomyces. The protocol outlines DNA amplification and gel electrophoresis to confirm a stable host-parasite interaction.
Fields of Study & Subjects
Oral microbiology and periodontitis research
Gut microbiota and health
Microbial infections and disease research
Publication details
- Journal / source
- Journal of Visualized Experiments
- Publication date
- 27 February 2026
- Language
- EN