Establishing Bacterial–Fungal Cross-Kingdom Biofilms for Studying Oral Pathogenesis

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Abstract

Source: Schlafer, S, and Frost Kristensen, M. Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy. J. Vis. Exp. (2020)This video demonstrates the cultivation of Streptococcus mutans and Candida albicans cross-kingdom biofilms under oral-like conditions to model bacterial–fungal interactions, enabling the study of microbial synergy, glucan-mediated adhesion, and acidogenic virulence in oral pathogenesis.

Fields of Study & Subjects

Oral microbiology and periodontitis research Bacterial biofilms and quorum sensing Antifungal resistance and susceptibility

Publication details

Journal / source
Journal of Visualized Experiments
Publication date
27 February 2026
Language
EN