Assessing Root Colonization of Tomato Seedlings by a Pathogenic Bacterium Using Viable Cell Counts

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Abstract

Source: Matthysse, A. G. Adherence of Bacteria to Plant Surfaces Measured in the Laboratory. J. Vis. Exp., (2018)This video demonstrates a protocol for measuring root colonization of tomato seedlings by wild-type and cellulose-deficient pathogenic bacteria. Viable counts of tightly adhering bacteria show greater adhesion and microcolony formation by the wild-type strain, highlighting cellulose’s role in stable root colonization.

Fields of Study & Subjects

Plant-Microbe Interactions and Immunity Plant Pathogenic Bacteria Studies Bacterial biofilms and quorum sensing

Publication details

Journal / source
Journal of Visualized Experiments
Publication date
27 February 2026
Language
EN