Immunostaining of Bacteria-Filled Microlesions in a Mouse Cardiac Tissue Section for Imaging

Source: Brown, A. O., and Orihuela, C. J. Visualization of Streptococcus pneumoniae within Cardiac Microlesions and Subsequent Cardiac Remodeling. J. Vis. Exp. (2015)The video demonstrates the immunostaining of chemically fixed mouse cardiac tissue sections to visualize pathogenic bacteria localized within infection-induced microlesions. The process includes tissue washing, membrane permeabilization, blocking of nonspecific sites, antibody labeling, nuclear counterstaining, and confocal microscopy to detect and localize bacterial clusters.