A Rapid Image-Based Assay for Screening Surface-Induced Bacterial Virulence

Journal of Visualized Experiments 2026

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DOI: 10.3791/24485
Cited by: 0

Abstract

Source: Perinbam, K., and Siryaporn, A. A Rapid Image-based Bacterial Virulence Assay Using Amoeba. J. Vis. Exp. (2018)This video demonstrates a rapid, image-based method to assess bacterial virulence by incubating amoebae with surface-attached or planktonic bacteria, applying a calcein-acetoxymethyl ester (calcein-AM) agarose pad, and imaging fluorescence to detect host cell damage.

Fields of Study & Subjects

Legionella and Acanthamoeba research Bacterial biofilms and quorum sensing Cell Image Analysis Techniques

Publication details

Journal / source
Journal of Visualized Experiments
Publication date
27 February 2026
Language
EN