Preparation of Bacterial Biofilm Colonies for Morphological Analysis

Journal of Visualized Experiments 2026

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DOI: 10.3791/24490
Cited by: 0

Abstract

Source: Bucher, T., et. al., Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors. J. Vis. Exp.(2016)This video demonstrates the preparation of mature bacterial biofilm colonies for high-resolution imaging. It covers fixation, dehydration, mounting, and metal coating steps to preserve and visualize biofilm morphology.

Fields of Study & Subjects

Bacterial biofilms and quorum sensing Biochemical and Structural Characterization Bacterial Genetics and Biotechnology

Publication details

Journal / source
Journal of Visualized Experiments
Publication date
27 February 2026
Language
EN