Protocol for Single-Cell Dissociation, RNA Sequencing, and Isolation of Myoepithelial Cells and Fibroblasts from the Aged Lacrimal and Salivary Glands

Anastasiia Ivanova; Vanessa Delcroix; Vina Dang; Anastasiya Kuznetsova; Neda Rashidi; Sicong Wong; Katja A. Lamia; Helen P. Makarenkova

doi:10.3791/70441

Protocol for Single-Cell Dissociation, RNA Sequencing, and Isolation of Myoepithelial Cells and Fibroblasts from the Aged Lacrimal and Salivary Glands

Journal of Visualized Experiments 2026

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Authors: Anastasiia Ivanova, Vanessa Delcroix, Vina Dang, Anastasiya Kuznetsova, Neda Rashidi, Sicong Wong, Katja A. Lamia, Helen P. Makarenkova

DOI: 10.3791/70441

Cited by: 1

Abstract

mice represented the fibroblast population. This protocol yields high-quality, viable single-cell suspensions from aged LGs and can be readily adapted to other fibrotic or aging exocrine tissues requiring gentle dissociation and targeted cell isolation.

Fields of Study & Subjects

Salivary Gland Tumors Diagnosis and Treatment Salivary Gland Disorders and Functions Congenital Ear and Nasal Anomalies

Publication details

Journal / source: Journal of Visualized Experiments
Publisher: MyJove Corporation
Issue: 230
Publication date: 24 April 2026
Language: EN