HPV E6/E7 mRNA vs. HPV DNA in co-testing with ThinPrep cytology for cervical cancer screening: a prospective observational diagnostic accuracy study of methylation biomarkers

Journal of Gynecologic Oncology 2026 Open access

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Authors: Xinling Zhao, Lei Chang, Wenyan Duan, Lijuan Liu, Yange Shi, Xiaoling Fan, Yu Bai, Yanli Wang, Jingya Li
DOI: 10.3802/jgo.2026.37.e109
Cited by: 1
References: 29

Abstract

OBJECTIVE: Human papillomavirus (HPV) gene transcription activity and methylation could be promising tools for cervical cancer (CC) screening. This study compared HPV E6/E7 messenger RNA (mRNA) + ThinPrep cytology test (TCT) vs. HPV genotyping + TCT in screening CC and explored the relationship between gene methylation and cervical lesions. METHODS: genes was performed on the biopsy specimens from 100 patients. RESULTS: genes increased with lesion severity. CONCLUSION: The sensitivity and specificity of HPV E6/E7 mRNA + TCT appeared higher than HPV DNA + TCT. Simultaneous positivity for HPV 16/18 and HPV E6/E7 mRNA suggests a high immediate risk of CIN2+ and CIN3+. These results may improve CC screening.

Fields of Study & Subjects

Cervical Cancer and HPV Research Endometrial and Cervical Cancer Treatments Reproductive tract infections research

Publication details

Journal / source
Journal of Gynecologic Oncology
Publisher
XMLink
Volume
37
Issue
5
Publication date
1 January 2026
Language
EN