Evaluation of Neuroprotective Effects of Ethanolic Leaf Extract from Causonis trifolia (L.) in AlCl3 Induced Alzheimer’s Disease in Wistar Rats

This study examined the neuroprotective effects of ethanolic leaf extract from Causonis trifolia (L.) on Alzheimer's disease in Wistar rats produced by aluminum chloride (AlCl3). Bioactive substances like 9-octadecanoic acid were found by phytochemical and GC-MS studies, and molecular docking supported their interaction with important targets for Alzheimer's disease. In vivo tests revealed that, in comparison to untreated disease controls, treatment with the extract at doses of 150 and 300 mg/kg, along with donepezil, significantly increased locomotor activity, decreased anxiety, and improved memory retention in behavioral tests (IR Actimeter, Elevated Plus Maze, and Novel Object Recognition). Furthermore, the extract reduced neurodegenerative damage in striatal tissue and restored antioxidant enzyme activity (SOD and CAT). All things considered, the results show that Causonis trifolia has encouraging anti-Alzheimer's properties, most likely due to its antioxidant processes.