Hypothermic machine perfusion protects DCD graft liver from ischemia‑reperfusion injury by enhancing macrophage efferocytosis via KLF2‑NLRP3 signaling

International Journal of Molecular Medicine 2026 Open access

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Authors: Qin Deng, Zhongzhong Liu, Qifa Ye, Jia Shuo Liu, ZhiHui Fu, Xingjian Zhang, Jun Luo, Zhongshan Lu, Pengpeng Yue, Jiansheng Xiao, Qi Xiao
DOI: 10.3892/ijmm.2026.5756
Cited by: 0

Abstract

. Furthermore, overexpression of KLF2 enhanced macrophage efferocytosis, whereas suppression of KLF2 impaired this. Moreover, enhanced efferocytosis contributed to inflammation resolution, ultimately improving overall graft injury and decreasing apoptosis. Mechanistically, KLF2 inhibited the NOD‑like receptor protein 3 (NLRP3) inflammasome to suppress pyroptosis, thereby indirectly enhancing efferocytosis. HMP alleviated IRI in DCD liver grafts by upregulating endothelial KLF2, which inhibited NLRP3 inflammasome‑mediated pyroptosis, thereby improving the inflammatory microenvironment and promoting macrophage efferocytosis.

Fields of Study & Subjects

Organ Transplantation Techniques and Outcomes Transplantation: Methods and Outcomes Phagocytosis and Immune Regulation

Publication details

Journal / source
International Journal of Molecular Medicine
Volume
57
Issue
4
Pages
1–18
Publication date
5 February 2026
Language
EN