Microbiome Modulation with Lactobacillus rhamnosus GG Potentiates Curcumin’s Efficacy in Reversing Gemcitabine Resistance of Gallbladder Cancer through Gut Microbiota-PI3K/AKT Axis
Abstract
) while reducing pathogenic taxa. LC-MS analysis further demonstrated restoration of bile acid homeostasis, characterized by elevated primary bile acids (GCA, CDCA) and decreased secondary bile acids (DCA, LCA). Mechanistically, the intervention significantly inhibited PI3K/AKT signaling, as confirmed by Western blot and immunohistochemistry. Bioinformatic analysis further identified PI3K/AKT as a central regulatory pathway. These findings indicate that probiotic-assisted CUR therapy reverses GEM resistance by remodeling the gut microbiota and its metabolic outputs, thereby suppressing oncogenic signaling pathways. This strategy provides a promising microbiota-based approach for improving therapeutic outcomes in GBC.
Fields of Study & Subjects
Curcumin's Biomedical Applications
Tannin
Tannase and Anticancer Activities
Cholangiocarcinoma and Gallbladder Cancer Studies
Publication details
- Journal / source
- Journal of Microbiology and Biotechnology
- Publisher
- Korean Society for Microbiology and Biotechnology
- Volume
- 36
- Pages
- e2601007–e2601007
- Publication date
- 21 April 2026
- Language
- EN