CRISPR-Cas9 Technology in Regenerative Medicine and Tissue Engineering

CRISPR/Cas9 technology has emerged as a revolutionary genome editing tool, offering unprecedented precision, efficiency, and versatility in manipulating genetic material. In the fields of regenerative medicine and tissue engineering, CRISPR/Cas9 holds transformative potential for correcting genetic defects, enhancing stem cell therapies, engineering tissue specific cellular functions, and developing disease models. This chapter explores the fundamental mechanisms of CRISPR/Cas9, its integration into regenerative strategies and its application in engineering functional tissues and organs. Key advancements include targeted gene editing in induced pluripotent stem cells (iPSCs), modulation of signaling pathways involved in tissue regeneration and creation of gene-edited scaffolds for organogenesis. The chapter also addresses the current challenges, such as off-target effects, ethical considerations, and regulatory hurdles, while highlighting emerging innovations and future directions for clinical translation. Overall, CRISPR/Cas9 stands at the forefront of next-generation therapeutic strategies, reshaping the landscape of personalized and regenerative medicine.