The Impact of Silver Nanoparticles on Small Intestine Cells and Oocytes Follicular Environment Cells in Female Mice

The impact of silver nanoparticles (AgNPs) on the intestine and reproductive system have not been sufficiently studied. Meanwhile, the relationship between disruption of the intestinal epithelial barrier and female reproductive disorders is under active investigation. The aim of the work was to evaluate the effects of AgNPs on small intestine cells and oocytes follicular environment cells under conditions of experimental premature ovarian failure (ePOF) and the use of Resveratrol in female mice. Methods . ePOF was modeled by immunizing with a suspension of kidney antigen (10 μl per 10 g of weight) according to the scheme. AgNPs (2,0 mg/kg) and Resveratrol (RES, 50,0 mg/kg) was administered intraperitoneally once a day three times after the last immunization.Enterocytes were isolated and cell death was assessed by the method of two-color fluorescent nucleic acid dye. Analyzed data with GraphPad Prism. Results . It was found that AgNPs under ePOF conditions did not cause significant changes in the viability of ileal enterocytes. In cells of the follicular environment of oocytes, under the influence of nanoparticles, a 1.40-fold decrease in the proportion of living cells and an increase in the proportion of cells with morphological signs of apoptosis and necrosis were observed, by 1.17 times and 1.43 times, respectively (p