Impact of semen quality and maternal age on blastocyst chromosomal abnormalities: a retrospective cohort study based on 1091 PGT-A cycles

In this study, we aimed to investigate the influence of paternal oligozoospermia (OZ), asthenozoospermia (AZ), or teratozoospermia (TZ) on blastocyst ploidy and its potential interaction with maternal age. We analyzed 3383 embryos in 1091 preimplantation genetic testing for aneuploidy (PGT-A) cycles via next-generation sequencing (NGS) at the Center for Reproductive Medicine, Shandong University (Jinan, China). Our findings revealed that compared to the normozoospermic (NZ) group, the AZ group exhibited significantly lower euploidy rates ( P = 0.022). This effect was more pronounced in cases with advanced maternal age (AMA; ≥38 years), where AZ patients had markedly reduced euploidy rates ( P = 0.003), while no significant differences were observed in younger women (