Evaluation of the effect of novel all-in-one irrigating solutions with and without diode laser activation on the radicular dentine composition: An in vitro attenuated total reflectance Fourier-transform infrared spectroscopy study

Background: Complete disinfection of the root canal while preserving dentinal integrity remains a major challenge. Diode laser activation improves irrigant penetration yet its combined effect with novel irrigants Triton and Twin Kleen on dentin composition remains unclear. Aims: To compare the effect of Triton, Twin Kleen, and sodium hypochlorite (5.25% NaOCl) +ethylenediaminetetraacetic acid (17% EDTA) with and without diode laser activation on the radicular dentin composition. Materials and Methods: = 20): Normal saline, Triton, Twin Kleen, and NaOCl + EDTA. Each group was further subdivided into diode laser-activated and nonactivated subgroups. Longitudinal dentin sections were analyzed using attenuated total reflectance Fourier-transform infrared spectroscopy to assess the root dentin inorganic and organic alterations. Statistical Analysis: < 0.05). Results: Among both laser and nonlaser activated groups, normal saline preserved the highest carbonate and amide peaks, followed by Triton and Twin Kleen and least in NaOCl + EDTA. Diode laser activation slightly decreased all FTIR ratios. Conclusion: Triton activated with and without diode laser, maintained dentin composition effectively, supporting its use as a single step irrigant in endodontic therapy.