Data supporting the publication: Structural remodelling and activation of the hdeAB and proVWX operons in response to pH and salt stress
Abstract
This is data supporting the paper titled, "Structural remodelling and activation of the hdeAB and proVWX operons in response to pH and salt stress"it consists of:1.Raw data files of RT-qPCR experiments of the hdeAB and proVWX operon and their flanking genes in the MG1655 ΔendA (WT,NT331), MG1655 ΔendA ΔstpA (ΔstpA, NT633), MG1655 ΔendA Δhns (Δhns, NT794), MG1655 ΔendA Δhns ΔstpA (Δhns ΔstpA, XT3236), MG1655 ΔendA Δhns ΔstpA ΔrpoS (Δhns ΔstpA ΔrpoS, XT3238), MG1655 ΔendA ΔrpoS (ΔrpoS, XT3132) in different pH (pH6.6/2.0)or osmotic condition (NaCl 0.08 M/ 0.08 M to 0.3M).2.Raw data files of ChIP-qPCR experiments of the hdeAB and proVWX operon and its flanking genes in the MG1655 ΔendA; hns::hns-3XFLAG (NT796), MG1655 ΔendA; stpA::stpA-3XFLAG (NT797), MG1655 ΔendA;hns::hns-3XFLAG; stpA::kanRccdB (NT866), MG1655ΔendA; stpA::stpA-3XFLAG; hns::kanRccdB (NT867) at pH 6.6 and pH 2.0.3.Raw data files of 3C-qPCR experiments of the hdeAB and proVWX operon and their flanking region in different pH (pH6.6/2.0)or osmotic condition (NaCl 0.08 M/ 0.08 M to 0.3M). For hdeAB operon, we use the transcription start site fragment (fragment 10 in our study) as the anchor fragment in MG1655 ΔendA (WT,NT331), MG1655 ΔendA ΔstpA (ΔstpA, NT633), MG1655 ΔendA Δhns (Δhns, NT794), MG1655 ΔendA Δhns ΔstpA (Δhns ΔstpA, XT3236), MG1655 ΔendA Δhns ΔstpA ΔrpoS (Δhns ΔstpA ΔrpoS, XT3238), MG1655 ΔendA ΔrpoS (ΔrpoS, XT3132).4.Agarose gel images in this study. 5.All the raw data used for figure generation in RT-qPCR, 3C-qPCR, ChIP-qPCR, MST, and bridging assays.
Fields of Study & Subjects
Bacterial Genetics and Biotechnology
Heat shock proteins research
Escherichia coli research studies
Publication details
- Journal / source
- 4TU.ResearchData
- Publication date
- 10 March 2026
- Language
- EN
- Document type
- Dataset