Data supporting the publication: Structural remodelling and activation of the hdeAB and proVWX operons in response to pH and salt stress

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Authors: Pingzhuang Ge, Fatema Zahra Rashid, Luuk Gaarthuis, Marc K M Cajili, Minkang Tan, Baoxu Pang, Karin Schnetz, Remus Thei Dame
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Abstract

This is data supporting the paper titled, "Structural remodelling and activation of the hdeAB and proVWX operons in response to pH and salt stress"it consists of:1.Raw data files of RT-qPCR experiments of the hdeAB and proVWX operon and their flanking genes in the MG1655 ΔendA (WT,NT331), MG1655 ΔendA ΔstpA (ΔstpA, NT633), MG1655 ΔendA Δhns (Δhns, NT794), MG1655 ΔendA Δhns ΔstpA (Δhns ΔstpA, XT3236), MG1655 ΔendA Δhns ΔstpA ΔrpoS (Δhns ΔstpA ΔrpoS, XT3238), MG1655 ΔendA ΔrpoS (ΔrpoS, XT3132) in different pH (pH6.6/2.0)or osmotic condition (NaCl 0.08 M/ 0.08 M to 0.3M).2.Raw data files of ChIP-qPCR experiments of the hdeAB and proVWX operon and its flanking genes in the MG1655 ΔendA; hns::hns-3XFLAG (NT796), MG1655 ΔendA; stpA::stpA-3XFLAG (NT797), MG1655 ΔendA;hns::hns-3XFLAG; stpA::kanRccdB (NT866), MG1655ΔendA; stpA::stpA-3XFLAG; hns::kanRccdB (NT867) at pH 6.6 and pH 2.0.3.Raw data files of 3C-qPCR experiments of the hdeAB and proVWX operon and their flanking region in different pH (pH6.6/2.0)or osmotic condition (NaCl 0.08 M/ 0.08 M to 0.3M). For hdeAB operon, we use the transcription start site fragment (fragment 10 in our study) as the anchor fragment in MG1655 ΔendA (WT,NT331), MG1655 ΔendA ΔstpA (ΔstpA, NT633), MG1655 ΔendA Δhns (Δhns, NT794), MG1655 ΔendA Δhns ΔstpA (Δhns ΔstpA, XT3236), MG1655 ΔendA Δhns ΔstpA ΔrpoS (Δhns ΔstpA ΔrpoS, XT3238), MG1655 ΔendA ΔrpoS (ΔrpoS, XT3132).4.Agarose gel images in this study. 5.All the raw data used for figure generation in RT-qPCR, 3C-qPCR, ChIP-qPCR, MST, and bridging assays.

Fields of Study & Subjects

Bacterial Genetics and Biotechnology Heat shock proteins research Escherichia coli research studies

Publication details

Journal / source
4TU.ResearchData
Publication date
10 March 2026
Language
EN
Document type
Dataset