Effect of Temperature, pH and Substrate Concentration on Bioethanol Yield in the Conversion of Plantain Peels into Bioethanol using Aspergillus niger and Saccharomyces cerevisiae

Interest in the development of biofuels has grown as a result of the global need for renewable and sustainable energy sources. Waste materials have a lot of promise as feedstock due to their abundance and low cost. This study evaluates the effect of temperature, pH and substrate concentration in the conversion of plantain peels into bioethanol. The treated biomass was concurrently saccharified and fermented using Aspergillus niger and Saccharomyces cerevisiae. By varying the substrate content between 5 and 10 weight percent, the pH between 4 and 10, and the temperature between 30 and 50°C, the relationship between substrate concentration, pH, temperature, and bioethanol yield was examined using the Box Behnken Design of Experiment (DOE). This project contributes to the development of renewable and sustainable energy sources by using waste materials as feedstock. The outcomes will have a big impact on environmental sustainability, waste management, and the creation of renewable energy. A spectrophotometer was used to measure the peels' ethanol output every 24 hours for seven days. At a pH of 5, a substrate concentration of 5 weight percent, and a temperature of 30 °C, a 5.7% concentration bioethanol solution was obtained out of 17 runs produced by Design Expert.