Extraction and wound healing activity of fucoidan loaded in-situ gel

Chronic Wound healing require sustained and targeted therapeutic strategies to enhance repair and regeneration. This study aimed to develop and evaluate a fucoidan-based in-situ gel formulation for enhanced wound healing through sustained and localized drug delivery. An in-situ gel system utilizing temperature- and pH-sensitive polymers such as poloxamers and chitosan was formulated with fucoidan extracted from undaria pinnatifida. The extracted fucoidan was characterized using UV-Visible and FTIR spectroscopy. Its wound healing efficacy was assessed using in vitro models. The formulation exhibited rapid gelation under physiological conditions, sustained drug release, and excellent biocompatibility. The fucoidan extract utilized in the in vitro wound scratch assay shows significant wound healing activity in L929 cell lines at a dose of 25µl. In L929 cells, the fucoidan extraction showed 26% wound-healing activity after 24 hours. Fucoidan-loaded gels significantly accelerated wound closure and enhanced tissue regeneration compared to controls