Ab initio Whole Cell Kinetic Model of Streptomyces murinus CR-43 (smuLA26)

Streptomyces species are known for the synthesis of bioactive compounds ranging from antibiotics, antifungals and enzymes with industrial value. Streptomyces murinus CR-43 has been shown to produce antinematodal fatty acids and may be potential for future metabolic engineering for fatty acid production. Mathematical kinetic models can inform and guide metabolic engineering strategies; however, there has been no whole-cell kinetic model of S. murinus CR-43 to-date. In this study, the ab initio whole-cell kinetic model of S. murinus CR-43 is constructed. Enzymatic genes were identified from the complete genome sequence of S. murinus CR-43 and mapped to metabolic reactions using KEGG enzyme nomenclature, enabling systematic reconstruction of the organism’s metabolic reactome. The resulting simulatable model, smuLA26, comprises of 1009 metabolites and 425 unique enzymes with 1385 enzymatic reactions. This model can be a preliminary framework for S. murinus CR-43, which serves as a platform for future refinement and exploration of metabolic engineering strategies targeting fatty acid and secondary metabolite production.