Phytochemical Screening and HPTLC Fingerprint Analysis of the Siddha Herbal Formulation Kadukkai Chooranam

The Siddha system of medicine is an indigenous traditional medical system that emphasizes preventive strategies, highlighting the principle that “Prevention is better than cure”. In Siddha literature particularly in Vaithiya Vilakkam Ennum Amirtha Saakaram, Kadukkai chooranam is described as a polyherbal formulation indicated for the management of Veezhi Akkaram. Aim: The aim of the study was to evaluate the phytochemical profile and HPTLC fingerprint analysis of Siddha polyherbal formulation Kadukkai Chooranam (KKC). Methodology: The formulation was subjected to standard qualitative phytochemical screening to identify various bioactive constituents. High Performance Thin Layer Chromatography (HPTLC) were performed using silica gel plates with appropriate solvent systems. The developed plates are examined under UV light at 366nm, and the chromatographic fingerprints were recorded. Results: The phytochemical screening of Kadukkai chooranam (KKC) revealed the presence of alkaloids, flavonoids, steroids, triterpenoids, coumarins, phenols, tannins, saponins, sugars, and betacyanin. HPTLC fingerprint analysis of the formulation revealed seventeen prominent peaks, indicating the presence of diverse phytochemical constituents. The major Rf values of the peaks were observed to range from 0.03 to 0.64. Conclusion: Based on the findings, it is concluded that it shows the compendious understanding of presence of phytochemical constituents and HPTLC fingerprint profile of Kadukkai chooranam (KKC) and it is competent to assess the quality profile of Kadukkai chooranam as a reference standard for the development of a standardized herbal pharmaceutical formulation.