Effect of Oodev (Oocyte Developer) Hormonal Induction on Estradiol, Vitellogenin and Cortisol Profiles of Putak Fish (Notopterus notopterus, Pallas 1769)

The putak fish (Notopterus notopterus) is a freshwater species native to Indonesia, protected due to its ecological importance.Oodev, a hormonal inducer, accelerates gonad maturation in the early stage.Estradiol and vitellogenin can serve as indicators of gonad maturation, while cortisol indicates stress response.This study aimed to profile estradiol, vitellogenin, and cortisol in putak fish induced with different doses of Oodev.A completely randomized design was used with four treatments and five replicates: control (0mL/kg), P1 (0.5mL/kg), P2 (0.7mL/kg), and P3 (0.9mL/kg).Twenty fish (19-25.6cmlength) were acclimatized for 30 days and treated for 90 days via intramuscular injection.Hormone profiles in blood plasma were measured using ELISA and analyzed by one-way ANOVA.Results showed the highest estradiol concentration in P1 (3450.01±47.05pg/mL)and the lowest in P2 (2886.73±214.73pg/mL).P2 was significantly lower than the control and P1 (P