Evaluation of Polymerase Chain Reaction Conditions and Primer Specificity for Plasmodium vivax 18S rRNA gene Detection Across Different Parasite Life Stages
Abstract
Introduction: Malaria caused by Plasmodium vivax remains widespread, with dormant liver stages that can trigger relapses. This study aimed to optimize denaturation and extension temperatures for qPCR detection of the P. vivax 18S rRNA gene, following previous optimization of primer concentration and annealing temperature.
Fields of Study & Subjects
Malaria Research and Control
Molecular Biology Techniques and Applications
Biosensors and Analytical Detection
Publication details
- Journal / source
- Gaceta Médica de Caracas
- Volume
- 134
- Issue
- S1
- Pages
- S125–S134
- Publication date
- 29 January 2026
- Language
- ES