VALIDATION AND OPTIMIZATION OF A PRIMER-BASED TEST SYSTEM FOR THE DETECTION OF THE ROOT-KNOT NEMATODE MELOIDOGYNE ENTEROLOBII
Abstract
This article focuses on the validation and optimization of a highly sensitive and specific real-time polymerase chain reaction (real-time PCR) method based on a test system employing species-specific primers and a fluorescent probe targeting the ITS2 region of ribosomal DNA (rDNA). This method enables rapid and accurate identification of the root-knot nematode Meloidogyne enterolobii and its differentiation from closely related species.
Fields of Study & Subjects
Nematode management and characterization studies
Helminth infection and control
Plant Pathogens and Fungal Diseases
Publication details
- Journal / source
- Plant Protection and Quarantine
- Issue
- 3
- Pages
- 28–31
- Publication date
- 1 January 2026
- Language
- EN