Study of Serum TNF-Α and Leptin Levels in Association with Insulin Resistance among Type 2 Diabetes Mellitus Patients

Background: Leptin is one of the important adipokines released from adipocytes, and its level increases with increasing body mass index (BMI).Tumor necrosis factor alpha (TNF-α) is a cytokine that is released by adipocytes and inflammatory cells in response to chronic inflammation, Type 2 diabetes mellitus (T2DM) is believed to be associated with low-grade chronic inflammation.Aim and Objective: To investigate the involvement of leptin and TNF-α in T2DM associated with obesity. Materials and Methods: ➢It is a retrospective -cross sectional study.➢ Sample size -200 (100-DM patients and 100 -Non Diabetic age matched controls) ➢ Medical records of and laboratory reports of 100 patients and 100 controls were retrieved and parameters were retrospectively viewed for clinical findings Inclusion: Patients with type II DM Patients were included Exclusion: The subjects with another metabolic syndrome (Were excluded) Collection of Blood Samples:• 5ml of blood samples were collected into a labeled heparinized bottles • Centrifuged and plasma was separated • Blood samples were used for the analysisResults and Discussion: Our study shows a significantly higher level of TNF-α in T2DM patients compared to controls In obese diabetic patients, the serum level of TNF-α showed ssignificantly increased in comparison with non-obese diabetic patients and obese non diabetic group.TNF-α was Ccorrelated positively with HbA1c.Conclusion: TNF-α is associated with concurrent obesity and T2DM and it was correlated with HbA1c.& it may suggest that TNF-α needs further investigation to explore if it has a role in monitoring the effectiveness of management in individuals with obesity and T2DM.