BCL-2 Gene Expression Effect on Haematological Profiles among CML Patients in Ile –Ife. Osun State. Nigeria

BCL-2 was the first anti-death gene. A key for oncogenic mechanism in several haematological malignancies, has been attributed to the upregulation of BCL-2 which is caused by numerous mechanisms. The aim of the study is to evaluate the BCL-2 gene mutation by its expression level and the resultant effect on haematological profiles among Chronic Myelogenous Leukemic (CML) patients in Ile Ife. This study was cross-sectional and a total of 100 consenting participants were recruited: 50 known CML patients and 50 control subjects. Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife, a Referral Centre for haematologic malignancies, and Seventh Day Adventist Hospital, Ile-Ife, were used as the study centers. Full blood count was done using 3-part haematology auto analyzer (Mindray), while RNA extraction and qPCR Bcl-2 quantification were done using real time PCR equipment. The results obtained from the study shows that there are higher expression levels of the Bcl-2 gene in CML samples compared with control samples, attaining significant p