INFLUENCE OF OZONE THERAPY ON MONONUCLEAR INFILTRATE AND MAST CELLS IN TISSUE REPAIR – IN VIVO PILOT STUDY

Objective: To evaluate the effect of ozone gas and ozonized oil on monomorphonuclear infiltrate and mast cell numbers in a standard experimental skin wound healing model. Methods: Thirty Wistar rats received a standardized skin wound and were assigned to three groups: control (CG), ozone gas insufflation (OGG), and topical sunflower ozonized oil (OOG). Euthanasia occurred on days 5 and 10. Histological sections were stained with Hematoxylin-eosin and toluidine blue for quantitative and semi-quantitative analyses. ANOVA followed by Student’s t-test was applied (p0.05). Mast cell counts, however, were significantly higher in ozone-treated groups, particularly degranulated mast cells on day 5 (OGG p=0.01; OOG p=0.02) compared to controls. Conclusion: Ozone therapy did not significantly modulate monomorphonuclear infiltrate but increased mast cell numbers during early wound healing, regardless of administration route.