Evaluation of Plasma Membrane Integrity of Sperm and Fertility of Post Thawed Frozen Semen of Black Bengal Goat

The study aimed to evaluate and compare the effectiveness of different freezing durations in combination with three semen diluents (Triladyl, Andromed, and Tris-citrate) on sperm plasma membrane integrity and fertility of post-thawed semen in Black Bengal goats. Data on frozen semen production and evaluation were collected over a period of six months. Post-thawed semen samples were obtained according to the specified freezing times and diluents, and sperm plasma membrane integrity was assessed using the Hypo-osmotic Swelling Test (HOST). In this study, 3 freezing times (F1 = Freezing time 15 minutes, F2 = Freezing time 20 minutes, and F3 = Freezing time 25 minutes) were used in an ice box filled with liquid nitrogen (LN) vapor. After freezing with 3 different freezing times and 3 diluters, F1 showed sperm motility ranged from 20.3±1.33% to 53.7±.88%. A significant effect (p