Replication Data for: Impact of infusing the cholesterol precursor mevalonate on the hypocholesterolemia of sepsis and its potential consequences: a paradoxical response

In a catheterized mouse model of cecal-ligation and puncture-induced sepsis, septic mice received either 5-day mevalonate infusion (78 mg/d) or placebo, versus healthy controls. Plasma HDL-, LDL-cholesterol, corticosterone, total bile acids, and adrenocortical lipids, myofiber cholesterol and muscle force were quantified. Expression markers of cholesterol homeostasis and structural integrity were investigated in adrenal, muscle and liver tissue. Liver mevalonate metabolites were quantified with LC-MS. Next, a secondary analysis on a prospective observational human study on the time course of adrenal function in the ICU was performed to assess the association between plasma cholesterol and cortisol and plasma mevalonate.