Preclinical Analysis of Bone Marrow-Derived Stem Cell Therapy Response and Transcriptomic Overlap Analysis in a Severe Alcoholic Hepatitis Mouse Model

Background/Aims: Severe alcoholic hepatitis (SAH) is a life-threatening form of alcoholic liver disease resulting in high short-term mortality.Mesenchymal stem cells (MSCs) have potent immunomodulatory effects and have been evaluated in various clinical trials for the treatment of chronic liver diseases.However, clinical evidence in patients with alcoholic hepatitis remains scarce, and the underlying mechanisms of MSCs in this population are not yet fully understood.Methods: An integrative meta-analysis identified conserved transcriptomic signatures of alcoholic hepatitis.These signatures were validated in an ethanol-induced murine model.A mouse model of SAH was induced via subacute ethanol exposure (5 g/kg) combined with thioacetamide injection.MSCs were administered at two concentrations (5×10⁵ or 1×10⁶ cells), depending on the treatment group.Results: In the animal model, MSCs treatment visibly alleviated liver injury induced by thioacetamide and ethanol.Significant reductions in tumor necrosis factor-α (p