Data from: Wolbachia uses ankyrin repeats to target specific fly proteins

Excel spreadsheet of full, raw mass spectrometry results from coimmunoprecipitation (coIP) experiments targeting two Wolbachia proteins - WARP754 and WARP434. We sought to identify native interactions between Wolbachia WARPs and host proteins using coIP. Towards that end, we generated polyclonal antibodies against full-length WARP434 and WARP754 and used the resulting sera, and pre-immune bleeds as controls, for a coIP using Drosophila JW18 cells infected with Wolbachia wMel. For both WARPs, we were able to pull down the target WARP in bulk lysate for the experimental conditions and identified top host protein targets present across all replicates, and either not present in our negative controls or significantly diminished in coverage. Interestingly, WARP754 antibody was able to pull down WARP434 by coIP, suggesting a possible interaction between these two proteins. That said, the WARP434 antibody did not reciprocally pull down WARP754, so this result should be cautiously interpreted.