Library transgenesis in zebrafish through delayed site-specific mosaic integration for in vivo pooled screening of transgenes

This dataset is from a study developing a zebrafish library transgenesis method using delayed site-specific integration to achieve single-transgene-per-cell mosaicism with high library diversity. The raw imaging data includes confocal Z-stacks (.nd2 files) of zebrafish larvae expressing a 2-component library of fluorescent proteins (GFP and mScarlet) in neurons, captured across multiple brain regions to assess transgene mutual exclusivity in mosaic animals. The raw sequencing data (.fastq files) include Illumina amplicon reads generated from genomic DNA of 12 mosaic larvae with integrated libraries of barcoded GFP constructs (6978-R2), as well as the injected source barcode library (7462-R1). Larvae were injected with a complex library of barcoded plasmids, enabling quantification of independent integration events.